SBB Resources

OMBRE Core for Osteocyte-mediated Bone Remodeling
We have developed new protocols for Tier 1 services to assess outcome measures of perilacunar remodeling, including:
1) lacunocanalicular staining – silver nitrate staining of histological sections and quantitative analysis of lacunocanalicular area
2) collagen organization – picrosirius red staining of collagen for polarized light microscopy and quantitative analysis of collagen linearity
3) PLR gene expression – qRT-PCR analysis of MMP2, MMP13, MMP14, and CatK gene expression in RNA isolated from bone samples
4) in vitro functional assay – quantitative analysis of intracellular pH, a PLR functional outcome, changes using the pH-sensitive fluorescent dye 5-(and-6)-carboxy-SNARF-1, AM

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Skeletal Phenotyping Handouts

Techniques in Skeletal Phenotyping

Sample Skeletal Analysis

Events

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Past Events: 

"Hands-on Skeletal Histomorphometry Workshop"
July 27, 2016, 1:00-4:30 PM
UCSF Mission Bay Campus, 1700 Owens Street, 4th Floor Seminar Room

Download slides from Dr. Wenhan Chang's presentation here.

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"Skeletal Phenotyping Workshop"
April 30, 2015, 3:00-5:00 PM
UCSF Mission Bay Campus, 1700 Owens Street, 4th Floor Seminar Room

Handouts provided during the workshop are available in electronic form above.