Wenhan Chang, PhD
Phone: 415-750-2089 ext. 3880
Email: [email protected]
Office: San Francisco VA Medical Center
Nanostring nCounter, led by Dr. Wenhan Chang, is located at the SF-VAMC Mission Bay campus at 1700 Owens Street.
Applications
Looking for a novel way to rapidly and quantitatively evaluate gene expression? The Nanostring nCounter fills the gap between qPCR and RNAseq by analyzing up to hundreds of transcripts at a time with small amounts of RNA. Nanostring uses fluorescent color-coded barcode probes and single molecule imaging to detect and count hundreds of unique transcripts in a single reaction at high sensitivity (< 1 copy per cell). Starting with 50 - 100ng total RNA, no enzymatic processing steps are required for gene expression analysis. The probes hybridize to approximately 100 bases, and can be multiplexed to detect up to 800 different targets in a single reaction. Pre-built CodeSets are commercially available, as well as custom UCSF Skeletal Biology custom CodeSets that survey 94 or 627 mouse genes implicated in musculoskeletal biology, including bone, cartilage, tendon and muscle. For inquiries, please contact Chia-Ling Tu at [email protected].
Available Codesets
- 94 gene codeset [PDF file download]
- 113 gene codeset [PDF file download]
- 627 gene codeset [PDF file download]
